Carmen Gianfrani
EMail081 - 6132224 Home Page Orcid Scholar Researchgate |
Carmen
Gianfrani
Institute of Biochemistry and Cell Biology Senior Researcher Nutrheff Thematic Biological validation
Research Interest and expertise in the field of nutraceuticals
and functional food
Keywords: Immune nutrition, cellular bioassay, plastic contaminants, in vitro and pre-clinical studies
Nutraceuticals and foods of interestMucosal Immunology and Immune Nutrition - Use of cellular bioassay based on human intestinal or blood T lymphocytes to assess: - immunostimulatory properties of dietary proteins. - in vitro and pre-clinical validation of enzymatic, biochemical and immunological strategies to detoxify food proteins. - impact of environmental contaminants, such as plastic derivatives, on human health, in particular on the differentiation, maturation and functionality of immune cells. gluten, cow’s milk proteins, hydrolized gluten containing food, polyphenols Fields of application Celiac disease, food allergy, immunetoxicity of food contaminants Facilities and equipments At IBBC are available: cells culture sterile facilities, light and fluorescent microscopes, flow cytometry (FACSCanto II, cell sorter FACSAria, Accuri C6 flow cytometer, BD), cell biology equipment, gamma-cells irradiator, ELISA and ELISPOT (AELVIS) readers, apparatus to perform molecular biology experiments (DNA Engine Opticon Real-Time PCR Detection System), ChemiDoc, VICTOR Nivo Multimode Microplate Reader. Mass Spectroscopy. At Pietro Castellino Research Area in Naples (AdRNA1) are also available: light microscopes (Zeiss LSM700, Zeiss Axio zoom, Apotome, Leica SP5, Nikon), electron microscopy that includes a FEI Tecnai G2 Spirit BT electron microscope, FEI Dual Beam SCIOS EM for automated 3D imaging/reconstruction, microtomes for sectioning and a sample preparation lab (Cryo ultra microtome, Ultra microtome), high throughput microscopy (Olympus Scan^R, Nikon, Robotic liquid handling stations. |
