Giuliana d’Ippolito
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Giuliana
d'Ippolito
Institute of Biomolecular Chemistry Senior Researcher Nutrheff Thematic Microbial technology, Biomolecular technologies (enzymatic, biochemical, molecular biology)
Research Interest and expertise in the field of nutraceuticals
and functional food
Keywords: microalgae; diatoms; biosynthesis; metabolism
Nutraceuticals and foods of interestResearch interest: "Exploitation of marine microalgae as functional food". Expertise: - cultivation of marine microalgae in bioreactors (0.1 ml-50L) under autotrophy and heterotrophy; - chemical and biochemical characterization of microalgal biomass; - isolation and structural elucidation of bioactive compounds; - biosynthesis and modulation of small molecules by using chemical and molecular approaches; - biotechnological development of sustainable bioprocesses. lipids, proteins, carbohydrates, bioactive molecules Fields of application protein-rich whole algae; lipid-rich whole algae; bakery products Facilities and equipments -Culture collection of about 30 marine microalgae -Cultivation systems for marine microrganisms (microalgae and hyperthermophilic bacteria) in autotrophic and heterotrophic conditions on small scale (0,1 ml-2L) and large scale (10-100 L) -Spectroscopy and elemental analysis: NMR spectrometer 400MHz – BRUKER; NMR spectrometer 400MHz – BRUKER Avance III CryoProbe Prodigy; NMR spectrometer 600MHz – BRUKER Avance III inverse TCI CryoProbe; FT-IR 4100 – JASCO; Spectrophotometer V-650 JASCO; Digital polarimeter P2000; Spectropolarimeter J-815; Spettrofotometers Beckman Coulter; CHNS Analyser Flash2000. -Mass spectrometry: Ion-trap GC–MS instrument, Thermo ITQ 700 Mass spectrometer interfaced with Thermo Focus GC Polaris Q Thermo Scientific; High resolution mass spectrometer Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific) on-line with a UHPLC apparatus Infinity 1290, Agilent; Micro-qToF mass spectrometer (Waters) with an electrospray ionization (ESI) source and coupled with a Waters Alliance HPLC system. -Chromatography: n° 4 HPLC equipped with PDA, ELSD, UV detector and autosampler. |
